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Why are there larger than expected bands after digestion with the BamHI-HF™ restriction enzyme?.How does the level of star activity of BamHI-HF compare to BamHI?.

What is the difference between BamHI-HF and BamHI?.

Is there any difference in the methylation sensitivity between BamHI-HF and BamHI?.

Is there a difference in cutting close to the ends between BamHI-HF and BamHI?.

The following reagents are supplied with this product: coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763) Reagents Supplied The resolution isĪt the single nucleotide level. Is determined by capillary electrophoresis and peak analysis. Restriction Enzyme Competitor Study: Nuclease ContaminationĮcoRI, NotI, and BamHI from multiple suppliers were tested in reactionsĬontaining a fluorescent labeled single stranded, double stranded blunt,ģ’overhang or 5’ overhang containing oligonucleotides. Of our HF restriction enzymes are shown below. Examples of nuclease contamination studies for some NEB extensively performs quality controls on all standard and high-fidelity Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. HF enzymes also exhibit dramatically reduced star activity. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing.